Objective: Self-disclosure plays an active role in the beginning of interpersonal communication, in the formation of intimacy, and in the trust of the communicators each other. In this study, it is aimed to examine the validity and reliability of the Turkish version of Self-Disclosure Scale. Methods: A total of 274 nursing and medical university students were included in this study between May-June 2016. The data were collected using The Sociodemographic Information Form and the Self-Disclosure Scale. Exploratory factor analysis with a varimax rotation was used to examine the construct validity. Reliability was evaluated using Cronbach a coefficients and item-total subscale correlations. The stability of the measures was examined through testretest reliability assessment. Results: As a result of exploratory factor analysis, five factors with 64.45% of the variance explained and an eigenvalue >1 were extracted. Factor loading of items are between 0.53 and 0.86. The Cronbachs alpha coefficient value for the scale is 0.70, and test-retest reliability coefficient value is 0.89. Conclusion: Self-Disclosure Scale is a valid and reliable instrument to evaluate self-disclosure level among university students. [Anadolu Psikiyatri Derg 2017; 18(6.000): 586-593]